abstract：:The species cowpox virus (CPXV), genus Orthopoxvirus (OPV), consists of isolates highly variable in their biological properties and their genotypes. A TaqMan PCR assay for the specific detection of CPXV DNA based on sequences of the ORF D11L has been developed recently. (Gavrilova et al., 2010; Shchelkunov et al., 201...

journal_title：Journal of virological methods

authors： Maksyutov RA,Gavrilova EV,Meyer H,Shchelkunov SN

更新日期：2015-01-01 00:00:00

abstract：:A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay has been developed to detect and differentiate between canine parvovirus (CPV) and mink enteritis virus (MEV). Eight CPV and three MEV epidemic strains isolated from 28 pathological samples from dogs and minks suspected of being infe...

journal_title：Journal of virological methods

authors： Zhang C,Yu Y,Yang H,Li G,Yu Z,Zhang H,Shan H

更新日期：2014-12-15 00:00:00

abstract：:Accurate detection and quantitation of viruses can be beneficial to plant-virus interaction studies. In this study, three SYBR green real-time RT-PCR assays were developed to quantitate grapevine leafroll-associated virus 3 (GLRaV-3) in infected vines. Three genomic regions (ORF1a, coat protein and 3'UTR) were targete...

journal_title：Journal of virological methods

authors： Bester R,Pepler PT,Burger JT,Maree HJ

更新日期：2014-12-15 00:00:00

abstract：:A specific padlock probe (PLP) and detection probe were designed to target the capsid protein gene of Taura syndrome virus (TSV), and a hyper-branched rolling circle amplification (HRCA) assay and a corresponding strip-based test were produced. The reaction time and temperature were optimized for both. The PLPs were l...

journal_title：Journal of virological methods

authors： Zhao Y,Yin W,Wang Y,Wang G,Li B

更新日期：2014-12-01 00:00:00

abstract：:A near-full genome genotypic assay for HCV1b was developed, which may prove useful to investigate antiviral drug resistance, given new combination therapies for HCV1 infection. The assay consists of three partially overlapping PCRs followed by Sanger population or Illumina next-generation sequencing. Seventy-seven the...

journal_title：Journal of virological methods

authors： Cuypers L,Snoeck J,Vrancken B,Kerremans L,Vuagniaux G,Verbeeck J,Nevens F,Camacho RJ,Vandamme AM,Van Dooren S

更新日期：2014-12-01 00:00:00

abstract：:The study of replication of viruses that require high bio-secure facilities can be accomplished with less stringent containment using non-infectious 'replicon' systems. The FMDV replicon system (pT7rep) reported by Mclnerney et al. (2000) was modified by the replacement of sequences encoding chloramphenicol acetyl-tra...

journal_title：Journal of virological methods

authors： Tulloch F,Pathania U,Luke GA,Nicholson J,Stonehouse NJ,Rowlands DJ,Jackson T,Tuthill T,Haas J,Lamond AI,Ryan MD

更新日期：2014-12-01 00:00:00

abstract：:Concurrent infection of pigs with two or more pathogens is common in pigs under intensive rearing conditions. Porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), Japanese encephalitis virus (JEV) and pseudorabies v...

journal_title：Journal of virological methods

authors： Rao P,Wu H,Jiang Y,Opriessnig T,Zheng X,Mo Y,Yang Z

更新日期：2014-11-01 00:00:00

abstract：:RT-qPCR, an established method for the detection of RNA viruses, requires internal RNA controls for the correct interpretation of PCR results. Robust and versatile RT-PCR controls can be achieved for example by packaging RNA into a virus-derived protein shell. In this study a MS2-based internal control system was deve...

journal_title：Journal of virological methods

authors： Felder E,Wölfel R

更新日期：2014-11-01 00:00:00

abstract：:A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1...

journal_title：Journal of virological methods

authors： Li J,Yu T,Zhou J,Tu W,Gao S,Liu X

更新日期：2014-11-01 00:00:00

abstract：:Infection with West Nile virus (WNV), a mosquito-borne flavivirus, is a growing public and animal health concern worldwide. Prevention, diagnosis and treatment strategies for the infection are urgently required. Recently, viral reverse genetic systems have been developed and applied to clinical WNV virology. We develo...

journal_title：Journal of virological methods

authors： Fernández IV,Okamoto N,Ito A,Fukuda M,Someya A,Nishino Y,Sasaki N,Maeda A

更新日期：2014-11-01 00:00:00

abstract：:Bovine viral diarrhea virus (BVDV) can contaminate biological products produced in bovine or porcine cells or manufactured using bovine sera. A rapid, specific, sensitive, and practical method of detecting BVDV in bio-products is needed. The purpose of this study was to compare three assays with respect to their abili...

journal_title：Journal of virological methods

authors： Zhang SQ,Tan B,Li P,Wang FX,Guo L,Yang Y,Sun N,Zhu HW,Wen YJ,Cheng SP

更新日期：2014-10-01 00:00:00

abstract：:Human cosaviruses (HCoSVs) are newly discovered viruses in Picornaviridae family. Until now, most published studies reported HCoSV detection using molecular techniques and genetic characterization of the virus. Nevertheless, no laboratory has yet reported the replication of these viruses in cultured cell lines. In the...

journal_title：Journal of virological methods

authors： Rezig D,Touzi H,Meddeb Z,Triki H

更新日期：2014-10-01 00:00:00

abstract：:Plum pox virus (PPV) causes the most destructive viral disease known as plum pox or Sharka disease in stone fruit trees. As an important regulated pathogen, detection of PPV is thus of critical importance to quarantine and eradication of the spreading disease. In this study, the innovative development of two AmplifyRP...

journal_title：Journal of virological methods

authors： Zhang S,Ravelonandro M,Russell P,McOwen N,Briard P,Bohannon S,Vrient A

更新日期：2014-10-01 00:00:00

abstract：:Bubaline herpesvirus 1 (BuHV1) is a member of ruminant alphaherpesviruses antigenically related to bovine herpesvirus 1 (BoHV1). The impact of BuHV1 infection in infectious bovine rhinotracheitis control program is difficult to establish, due to the lack of specific diagnostic test. The ectodomain of glycoprotein E of...

journal_title：Journal of virological methods

authors： Nogarol C,Bertolotti L,De Carlo E,Masoero L,Caruso C,Profiti M,Martucciello A,Galiero G,Cordioli P,Lelli D,Nardelli S,Ingravalle F,Rosati S

更新日期：2014-10-01 00:00:00

abstract：:A RT-PCR approach for the direct detection and typing of human enteroviruses in the environment is described in this study. A semi-nested RT-PCR using COnsensus-DEgenerated Hybrid Oligonucleotide Primers (CODEHOP) designed from the VP2 genome region has been developed for the direct typing of enteroviruses in clinical...

journal_title：Journal of virological methods

authors： Ibrahim W,Ouerdani I,Pillet S,Aouni M,Pozzetto B,Harrath R

更新日期：2014-10-01 00:00:00

abstract：:Advances in massively parallel DNA sequencing platforms, commonly termed next-generation sequencing (NGS) technologies, have greatly reduced time, labor, and cost associated with DNA sequencing. Thus, NGS has become a routine tool for new viral pathogen discovery and will likely become the standard for routine laborat...

journal_title：Journal of virological methods

authors： Ip HS,Wiley MR,Long R,Palacios G,Shearn-Bochsler V,Whitehouse CA

更新日期：2014-09-01 00:00:00

abstract：:Cyprinid herpesvirus-3 (CyHV-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding...

journal_title：Journal of virological methods

authors： Gotesman M,Soliman H,Besch R,El-Matbouli M

更新日期：2014-09-01 00:00:00

abstract：:Shrimp farming is one of the most important aquaculture activities. Expansion and intensification of shrimp farming has been accompanied with the outbreak of diseases, which threaten the development and sustainability of the industry. Viral diseases are the major challenges faced by shrimp farming industries. The prev...

journal_title：Journal of virological methods

authors： Ramesh Kumar D,Sanjuktha M,Rajan JJ,Ananda Bharathi R,Santiago TC,Alavandi SV,Poornima M

更新日期：2014-09-01 00:00:00

abstract：:Serologic diagnosis of human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) infections has been shown to complement virus detection methods in epidemiologic studies. Enzyme immunoassays (EIAs) using cultured virus lysate antigens are often used to diagnose infection by demonstration of a ≥4-fold r...

journal_title：Journal of virological methods

authors： Zhang Y,Brooks WA,Goswami D,Rahman M,Luby SP,Erdman DD

更新日期：2014-09-01 00:00:00

abstract：:The major capsid protein (MCP) is a main structural protein of iridoviruses, and is used as a marker for the identification, differentiation and classification of ranaviruses. In the present study, six monoclonal antibodies (mAbs) against recombinant MCP of grouper iridovirus (GIV) were produced and characterized. All...

journal_title：Journal of virological methods

authors： Lin HY,Liou CJ,Cheng YH,Hsu HC,Yiu JC,Chiou PP,Lai YS

更新日期：2014-09-01 00:00:00

abstract：:Six single-chain fragment variable (scFv) antibodies against infectious haematopoietic necrosis virus (IHNV) were selected from an antibody phage display library by phage display technology. The soluble scFv antibodies showed a molecular weight 32kDa by Western blot. Dot blot analysis revealed that the six scFv antibo...

journal_title：Journal of virological methods

authors： Liu H,Zheng X,Shi X,Yu L,Jia P,Wang J,He J,Lan W,Liu H,Wu Z

更新日期：2014-09-01 00:00:00

abstract：:The human coronavirus NL63 was identified in 2004 and subsequent studies showed its worldwide distribution. Infection with this pathogen is associated with upper and lower respiratory tract diseases of mild to moderate severity. Furthermore, HCoV-NL63 is the main cause of croup in children. Within this study an optima...

journal_title：Journal of virological methods

authors： Florek D,Burmistrz M,Potempa J,Pyrc K

更新日期：2014-09-01 00:00:00

abstract：:The ferret is an excellent model for many human infectious diseases including influenza, SARS-CoV, henipavirus and pneumococcal infections. The ferret is also used to study cystic fibrosis and various cancers, as well as reproductive biology and physiology. However, the range of reagents available to measure the ferre...

journal_title：Journal of virological methods

authors： Carolan LA,Butler J,Rockman S,Guarnaccia T,Hurt AC,Reading P,Kelso A,Barr I,Laurie KL

更新日期：2014-09-01 00:00:00

abstract：:The performance of the rapid confirmatory HIV 1/2 assay Geenius was compared with the conventional HIV 1/2 line immunoblot (INNO-LIA HIV I/II Score). One hundred HIV 1/2 confirmed positive samples from donors and patients and 136 negative screening samples from blood donors were evaluated with both assays. A 20 member...

journal_title：Journal of virological methods

authors： Tinguely C,Schild-Spycher T,Bahador Z,Gowland P,Stolz M,Niederhauser C

更新日期：2014-09-01 00:00:00

abstract：:Sensitive, precise and rapid detection tests are needed in the quality control of rabies vaccine for rabies virus nucleoprotein. Previous studies for quantitation of rabies virus nucleoprotein focused on enzyme-linked immunosorbent assay (ELISA). A novel immunoassay for rapid determination of rabies virus nucleoprotei...

journal_title：Journal of virological methods

authors： Lin G,Huang H,Liu T,He C,Liu J,Chen S,Hou J,Ren Z,Dong W,Wu Y

更新日期：2014-09-01 00:00:00

abstract：:Hepatitis B virus (HBV) is hyperendemic to southern Africa, with genotype A of HBV being the predominant genotype, and subgenotype A1 prevailing. Infection with this subgenotype is associated with rapid disease progression, and high frequency of hepatocellular carcinoma development. The objectives of our study was to ...

journal_title：Journal of virological methods

authors： Bhoola NH,Reumann K,Kew MC,Will H,Kramvis A

更新日期：2014-07-01 00:00:00

abstract：:Varicella zoster virus (VZV) PCR is highly sensitive compared to traditional detection methods like culture and direct fluorescent antibody testing (DFA); however, the high cost of commercial assays prohibits their use in many clinical laboratories. Major contributors to cost are the nucleic acid extraction and the PC...

journal_title：Journal of virological methods

authors： Binkhamis K,Al-Siyabi T,Heinstein C,Hatchette TF,LeBlanc JJ

更新日期：2014-06-01 00:00:00

abstract：:Droplet Digital PCR (ddPCR) represents a new and alternative platform to conventional quantitative-PCR (qPCR) for the quantitation of DNA templates. However, the proposed improvement in sensitivity and reproducibility offered by ddPCR is not yet fully proven, partly because the delineation between positive and negativ...

journal_title：Journal of virological methods

authors： Jones M,Williams J,Gärtner K,Phillips R,Hurst J,Frater J

更新日期：2014-06-01 00:00:00

abstract：:Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and ...

journal_title：Journal of virological methods

authors： Chang HH,Chen PK,Lin GL,Wang CJ,Liao CH,Hsiao YC,Dong JH,Sun DS

更新日期：2014-06-01 00:00:00

abstract：:In vitro studies related to various viral pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. The susceptibility to porcine rotavirus infection was evaluated by using a newly established porcine intestinal epithelial cell line. Immunohistochemical staining for cytokeratin confirmed tha...

journal_title：Journal of virological methods

authors： Wang J,Hu G,Gao W,Xu L,Ning P,Zhang Y

更新日期：2014-06-01 00:00:00

abstract：:The development of rapid and simple gene amplification tests is required for detection of pathogens to prevent transmission of infectious diseases between animals or from animals to humans. An easy-to-use rapid gene amplification method that can directly detect RNA and DNA viruses in clinical samples was developed. Th...

journal_title：Journal of virological methods

authors： Segawa T,Kobayashi Y,Sase Y,Itou T,Suzuki M,Endoh T,Nakanishi T,Sakai T

更新日期：2014-06-01 00:00:00

abstract：:Emergence of lamivudine (LAM) resistance causes treatment failure in patients with chronic hepatitis B and compromise the efficacy of subsequent salvage therapies with other nucleot(s)ide analogs (NAs). Establishment of cell-based assays supporting LAM-resistant hepatitis B virus (HBV) replication will not only provid...

journal_title：Journal of virological methods

authors： Zhang Y,Zhang Y,Kang Y,Wang J,Liu H,Zhu H,Qin Y,Mao R,Lin X,Lu M,Zhang J

更新日期：2014-06-01 00:00:00

abstract：:A real-time TaqMan RT-PCR assay was developed for the rapid and sensitive detection of Tomato ringspot virus (ToRSV), an important plant virus which infects a wide range of fruit and ornamental crops. Primers and a probe were designed based on the highly conserved 3'-untranslated region (UTR) sequences of ToRSV, to am...

journal_title：Journal of virological methods

authors： Tang J,Khan S,Delmiglio C,Ward LI

更新日期：2014-06-01 00:00:00

abstract：:Tomato necrotic stunt virus (ToNStV) is an emerging potyvirus that causes severe stunting to infected tomato plants. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for sensitive detection of ToNStV. The sensitivity of RT-LAMP was comparable to that of conventional RT-PCR, ...

journal_title：Journal of virological methods

authors： Li R,Ling KS

更新日期：2014-05-01 00:00:00

abstract：:Cutting edge immune monitoring techniques increasingly measure multiple functional outputs for various cell types, such as intracellular cytokine staining (ICS) assays that measure cytokines expressed by T cells. To date, however, there is no precise method to measure virus-specific cytokine production by both T cells...

journal_title：Journal of virological methods

authors： Pattacini L,Murnane PM,Fluharty TR,Katabira E,De Rosa SC,Baeten JM,Lund JM

更新日期：2014-04-01 00:00:00

abstract：:An automated blotter was evaluated for detection of the HPV SPF10-LiPA25 RHA for HPV genotyping with 63 FFPE tissue and 45 WHO proficiency panel samples. The results showed that 10-20% more types were detected and bands were of significantly stronger intensity (p<0.0001) compared to manual processing. Therefore it was...

journal_title：Journal of virological methods

authors： Cornall AM,Quint WH,Garland SM,Tabrizi SN

更新日期：2014-04-01 00:00:00

abstract：:West Nile virus (WNV), genus Flavivirus, family Flaviviridae, is a major cause of viral encephalitis with broad host range and global spread. The virus has undergone a series of evolutionary changes with emergence of various genotypic lineages that are known to differ in type and severity of the diseases caused. Curre...

journal_title：Journal of virological methods

authors： Kolekar P,Hake N,Kale M,Kulkarni-Kale U

更新日期：2014-03-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63°C and 60min, respectively. LAMP amplicons...

journal_title：Journal of virological methods

authors： Chen MH,Kuo ST,Renault T,Chang PH

更新日期：2014-02-01 00:00:00

abstract：:Mammalian orthoreoviruses (reoviruses) have served as highly useful models for studies of virus replication and pathogenesis. The development of a plasmid-based reverse genetics system represented a major breakthrough in reovirus research. The current reverse genetics systems for reoviruses rely on the expression of T...

journal_title：Journal of virological methods

authors： Komoto S,Kawagishi T,Kobayashi T,Ikizler M,Iskarpatyoti J,Dermody TS,Taniguchi K

更新日期：2014-02-01 00:00:00

abstract：:A multiplex polymerase chain reaction (mPCR) was developed and evaluated for detection of pox viral infections simultaneously using clinical samples from sheep and goats. Specific primers for three pox viruses of sheep and goats including sheeppox virus (SPPV), goatpox virus (GTPV) and orf virus (ORFV) were designed t...

journal_title：Journal of virological methods

authors： Venkatesan G,Balamurugan V,Bhanuprakash V

更新日期：2014-01-01 00:00:00